September 16, 2020
Supplementary MaterialsFIG?S1. FIG?S2. Phylogenetic analysis based on 6,890 core SNVs of O89b-made up of genomes and assemblies. A phylogenetic tree was constructed by the maximum likelihood method in the MEGA7 program using 6,890 core SNVs of the O89b-made up of strains, plus MG1655. Sequence types are indicated. Download FIG?S2, PDF file, 0.02 MB. Copyright ? 2019 Zeng et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. TABLE?S2. genomes carrying the 69.2-kb insertion. Download Table?S2, PDF file, 0.1 MB. Copyright ? 2019 Zeng et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S3. Growth inhibition assay of Sanji compared to TOP10. Overnight cultures of TOP10 and Sanji were adjusted to an optical density at 600 nm (OD600) of 10 with fresh LB broth. Cultures (5 l) of TOP10 alone were spotted onto an agar plate (A), and cultures of a mixture Synaptamide of TOP10 and Sanji were spotted onto individual agar plates at a ratio of 100:1 (B), 10:1 (C), or 1:1 (D). After incubation at 37C for 2 hours, the agar discs made up of the cells were excised and resuspended in 2 ml of LB broth. The resuspended cells were diluted 104-fold with LB broth, and 100 l of this dilution was plated and incubated at 37C overnight and then at room heat afterward. The green colonies were visible after 3 days. The image shown was taken 12 days after plating. Download FIG?S3, PDF file, 1.6 MB. Copyright ? 2019 Zeng et al. This content is distributed under the Synaptamide terms of the Creative Commons Attribution 4.0 International license. TABLE?S3. Antibiotic resistance gene profiles of pSJ_255-related IncHI2 plasmids. Download Table?S3, PDF file, 0.1 MB. Copyright ? 2019 Zeng et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S4. BRIG circular gene plot comparison of plasmids related to pSJ_82. The reference sequence is a composite generated by inserting sequences within various other plasmids but absent in pSJ_82 in to the pSJ_82 series. Each band corresponds to a new plasmid the following from internal to Synaptamide external band: pSJ_82 represents plasmid pSJ_82 from Sanji; pKP04CTXM from Fight2D2; RCS62_pI from stress; tig00008015 from Sanji; AR_0011 represents plasmid tig00001011_pilon from AR_0011; AR_0014 represents plasmid unitig_1_pilon from AR_0014; pCREC-532_1 represents plasmid pCREC-532_1 from CREC-532; pCREC-629_1 represents plasmid pCREC-629_1 from CREC-629; AR_0150 represents plasmid tig00000255 from AR_051; p1493-5 represents plasmid p1493-5 from CRE1493; pECY55 represents plasmid from Y5; FDAARGOS_434 represents plasmid unnamed1 from FDAARGOS_434; and SCEC020007 represents plasmid pNDM5_0200007 from SCEC020007. All identifiable antibiotic level of resistance genes are tagged in red in the external band. Download FIG?S5, PDF file, Synaptamide 0.4 MB. Copyright ? 2019 Zeng et al. This article is distributed beneath the conditions of the Innovative Commons Attribution 4.0 International permit. FIG?S6. Operon framework of Tninto Is certainly26 components in ST167 strains. Plasmids p1493-5 from CRE1493, pECY55 from Y5, and unnamed1 from FDAARGOS_434 include a gene cluster flanked by ISelements, while plasmids unitig_1_pilon from tig00001011_pilon and AR_0014 from AR_0011 come with an gene aswell. The genes are indicated in cyan; ISelements in magenta; and Tntransposase genes in green. Download FIG?S6, PDF document, 0.1 MB. Copyright ? 2019 Zeng et al. This article is distributed beneath the conditions of the Innovative Commons Attribution 4.0 International permit. FIG?S7. BRIG round gene plot evaluation of plasmids linked to pSJ_98. The guide series is a amalgamated generated by placing sequences within various other plasmids but absent in pSJ_98 in to the pSJ_98 series. Each band corresponds to a new plasmid Sincalide the following from internal to external band: pSJ_98 represents plasmid pSJ_98 from Sanji; p1493-4 represents plasmid p1493-4 from CRE1493; pCREC-629_2 represents plasmid pCREC-629_2 from CREC-629; pBJ114-96 represents plasmid pBJ114-96 from BJ114; p1303_95 represents plasmid p1303_95 from 1303; pMS6198C represents plasmid pMS6198C from WE-0250. The gene may be the just antibiotic level of resistance gene seen in one person in this plasmid family members and is tagged in reddish colored. Download FIG?S7,.