Moreover, to our best knowledge, no outbreaks of HAdV16 contamination have ever been reported in Hunan province, China

Moreover, to our best knowledge, no outbreaks of HAdV16 contamination have ever been reported in Hunan province, China. of anti-HAdV4 nAb and anti-HAdV7 nAb increase with age, with individuals more youthful than 20 exhibiting the lowest seropositivity rates. Both seropositivity rates and nAb titers are comparable between different sex groups. Notably, HAdV4-seropositive individuals tend to be HAdV7-seropositive and 0.01; Supplementary Furniture S2, S3). In the whole cohort, HAdV4-seropositive individuals had significantly higher MEN2B nAb titers than HAdV7-seropositive ones (Mann-Whitney test, 0.001, Figure ?Physique2A).2A). According to nAb titers, the serum samples were categorized into four subgroups: unfavorable, 72; low, 72C200; moderate, 201C1000; and high, 1000. The distributions of different nAb titers were analyzed (Physique ?(Figure2B).2B). 34.0% of serum samples (95% CI 32.6C38.0%) contained high nAb titers to HAdV4, whereas only 22.9% (95% CI 21.9C26.9%) of serum samples experienced high nAb titers to HAdV7 (Chi-square test, 0.001). Significantly more serum samples contained moderate nAb titers to HAdV7 (26.8%; 95% CI 24.4C29.2%) than to HAdV4 (16.3%; 95% CI 14.3C18.3%) (Chi-square test, 0.001, Figure ?Physique2B).2B). These results suggested that even though seropositivity rate of anti-HAdV4 nAb was lower than that of anti-HAdV7 nAb, HAdV4-seropositive individuals tended to have higher nAb titers in comparison with HAdV7-seropositive individuals. Open in a separate window Physique 2 The overall seroprevalence of anti-HAdV4 nAb and anti-HAdV7 nAb. (A) The nAb titers against DBeq HAdV4 and HAdV7 in seropositive individuals. The data was analyzed with the Mann-Whitney test. ??? 0.001. (B) The distributions of anti-HAdV4 nAb DBeq and anti-HAdV7 nAb titers. nAb titers were classified into four subgroups: unfavorable, 72; low, 72C200; moderate, 201C1000; and high, 1000. The percentages of individuals with unfavorable, low, moderate, and high nAb titers were shown. Seropositivity Rates but Not Titers of Anti-HAdV4 nAb and Anti-HAdV7 nAb Increased With Age We analyzed the styles of seroprevalence in different age groups. The overall seropositivity rates of anti-HAdV4 nAb and anti-HAdV7 nAb increased with age (Chi-square test for pattern, HAdV4: 0.001; HAdV7: 0.001; Physique ?Physique3A).3A). The frequency of serum samples with moderate and high anti-HAdV4 nAb and anti-HAdV7 nAb titers also progressively increased with age (Figures 3B,C). However, the frequency of serum samples with low anti-HAdV4 nAb and anti-HAdV7 nAb titers increased with age before 50 but decreased thereafter (Figures 3B,C). Notably, in each age group, the percentage of individuals with high anti-HAdV4 nAb titers was higher than that with moderate and low anti-HAdV4 nAb titers (Physique ?(Physique3B),3B), whereas individuals with moderate anti-HAdV7 nAb titers dominated in HAdV7-seropositive ones (Physique ?(Physique3C).3C). The titers of anti-HAdV4 nAb was higher in the age group more youthful than 20 than the other age groups (Kruskal-Wallis test, 0.001; Physique ?Physique3D).3D). However, no difference was observed for the titers of anti-HAdV7 nAbs among age groups (Kruskal-Wallis test, = 0.399; Physique ?Physique3E).3E). These results revealed a different distribution of seropositivity rates as well as nAb titers for HAdV4 and HAdV7, and implied that individuals more youthful than 20 may be more susceptible to HAdV4 and HAdV7 contamination than older individuals because of relatively lower nAb seropositivity rates. Open in a separate window Physique 3 Seroprevalence and titer distribution of anti-HAdV4 nAb and anti-HAdV7 nAb in different age groups. (A) The seropositivity rates of anti-HAdV4 nAb and anti-HAdV7 nAb in different age groups. The data were analyzed with Chi-square test DBeq for pattern. (B,C) The distributions of the low (72C200), moderate (201C1000), and high ( 1000) titers of anti-HAdV4 nAb (B) and anti-HAdV7 nAb (C) in different age groups. (D,E) The titers of nAb to HAdV4 (D) and HAdV7 (E) in different age groups. The data was analyzed with the Kruskal-Wallis test. ??? 0.001. Seropositivity Rates and Titers of Anti-HAdV4 nAb and Anti-HAdV7 nAb Were Not Affected by Sex We next analyzed the seroprevalence and titer distributions of anti-HAdV4 nAb and anti-HAdV7 nAb in sex groups. The seropositivity rates of anti-HAdV4 nAb in males was slightly higher than that in.