Tag: Rabbit Polyclonal to SCNN1D

Some studies have shown the usability of neoadjuvant chemotherapy (NAC) in gastric cancer (GC). impartial predictor of response (= 0.04). However, in the paclitaxel/cisplatin group, no variables were associated with response. Taken together, our results suggest that OCT2high may represent a potential predictor of response to NAC with S-1/cisplatin in gastric cancer. hybridization (FISH), respectively. Tumors with strong positive staining in at least one cancer cell cluster (five or more cells) or FISH ratio of > 2.2 were regarded as positive for HER2. buy 1223001-51-1 Immunohistochemistry of OCT2 Sections of biopsy specimens were deparaffinized in xylene and then immersed in a graded alcohol series, and rehydrated in tap water. Heat-induced antigen retrieval (HIAR) method was applied using a pressure cooker for buy 1223001-51-1 10 min at 120C in 0.001 mol/L EDTA (pH 8.0). After HIAR, the sections were cooled to room temperature (RT) for 30 min. Sections were then rinsed in tap water followed by phosphate-buffered saline (PBS; pH 7.2). The sections were subsequently incubated with an anti-human OCT2 rabbit polyclonal antibody (1:800 dilution; Atlas Antibodies, Stockholm, Sweden) overnight at RT. After rinsing with PBS, sections were incubated with the Histofine Simple Stain MAX-PO (Nichirei Bioscience, Tokyo, Japan) as a secondary reagent for 1 h at RT. Thereafter, reaction products were developed using diaminobenzidine solution (Dako, Glostrup, Denmark) and the nuclei were lightly counterstained with Mayers hematoxylin. A negative control was included in each run without Rabbit Polyclonal to SCNN1D applying the primary antibodies. Sections of normal kidney were used as a positive control. Assessment of buy 1223001-51-1 OCT2 level All of the immunostaining results were assessed by three investigators (A.N., R.T., and S.K.) who had no knowledge of the clinicopathologic details of the patients. The staining intensity on cell membranes was graded on a scale of 0-3 (0, none; 1, weak; 2, moderate; 3, strong). The percentage of positive tumor cells was scored as follows: 0, 0%; 1, 1-10%; 2, 11-50%; 3, 51-100%. A final semi-quantitative score (0-6) was derived by adding the intensity score and the percentage score. According to the cutoff score determined by receiver operating characteristic curve analysis, the cutoff threshold was set at 4 using the best sensitivity and specificity. Scores of 0-3 were considered low expression level (OCT2low) and scores of 4-6 were considered high expression level (OCT2high). In the event of disagreement, the three investigators reevaluated the immunostained sections and discussed the interpretation until agreement was reached. Statistical analysis The Fishers exact test was used to evaluate the association of OCT2 level with patient age and sex, tumor localization, Laurn classification, histologic grade, HER2 status, and NAC regimen. The Fishers exact test was also used to determine the association of pathologic response with patient age and sex, tumor localization, Laurn classification, histologic grade, HER2 status, NAC regimen, and OCT2 level. A logistic regression model for multivariate analysis buy 1223001-51-1 was performed to identify impartial predictors of response. All variables assessed around the univariate analysis were included in the multivariate analysis. Differences with a = 0.03) and low histologic grade (= 0.03). However, no significant association with OCT2 level was detected for age, sex, tumor localization, HER2 status, or NAC regimen. Table 2 Association between OCT2 level and clinicopathologic parameters Univariate analysis of association of clinicopathologic parameters or OCT2 level with pathologic response to NAC in the entire cohort.