The responses of cells to chemical signals are well characterized and
May 26, 2017
The responses of cells to chemical signals are well characterized and understood relatively. general principles by which they work are beginning to be revealed. This Commentary highlights selected recent reports of mechanical signaling during disease development discusses open questions regarding the physical mechanisms by which cells sense stiffness and examines the relationship between studies in vitro on flat substrates and the more complex three-dimensional setting in vivo. Keywords: Cell mechanics Matrix stiffness Mechanosensing Introduction If we probed our environment using only those senses that rely on biochemical signaling that is smell and taste we would be highly limited in the amount and type of information we could process and would therefore BIBW2992 probably make decisions with undesirable and ultimately devastating consequences. Such a limited repertoire of stimuli and information is usually imposed around the cell if biology is usually defined solely by the signals a cell receives from molecules that bind in specific ways to its receptors by the intracellular biochemical reactions that are brought on by these binding events and by the direct biochemical consequences of genetically encoded information. Specificity and high affinity of molecular interactions are sometimes perceived as hallmarks of biological relevance as though evolution has devoted specific efforts to create structures and interactions that overwhelm ‘non-specific’ physical effects such as electrostatic and mechanical forces rather than exploit these forces to direct biological function within different cells and organisms. However with the continuing growth BIBW2992 of obtainable genomic and proteomic details increasing evidence implies Rabbit Polyclonal to IKK-gamma (phospho-Ser31). that genetics and biochemistry by itself are not enough to explain essential natural phenomena. Including the observations that different cell types possess different shapes which different organs possess different rigidity two elements of fundamental importance for diagnosing disease or analyzing wound recovery and embryonic advancement cannot be described in solely biochemical conditions. Furthermore the mechanised environment of the cell determines not merely its mechanised BIBW2992 properties such as for example rigidity and contractility but also its phenotype. Despite exceptional progress in genetics biochemistry and cell biology medical diagnoses are still routinely based on how a cells feels how it blocks radiation and how it yields to a knife. The properties of cells and cells that show useful in analysis further demonstrate the fundamental importance of physical factors in biology. Among the BIBW2992 features that differentiate normal from diseased cells and cells is definitely often tightness which is generally quantified as an elastic modulus; see Package 1 and Buxboim et al. (Buxboim et al. 2010 Chen (Chen 2008 and Janmey and Schliwa (Janmey and Schliwa 2008 for summaries of how soft-matter mechanics and terminology apply to cell biology. It remains to be verified whether a change in cells stiffness is merely a consequence of disease or also a contributing and even initiating factor in its development (Georges et al. 2007 Levental et al. 2007 Levental et al. 2009 However several lines of evidence suggest that matrix and cell mechanics can act as powerful signals for control of the cell cycle (Klein et al. 2009 Winer et al. 2009 initiation of specific transcription programs (Engler et al. 2007 Li et al. BIBW2992 2007 and development of organ dysfunction (Georges et al. 2007 Package 1. Terminology of cell and cells mechanics Stress. The pressure exerted on an object normalized by the area over which the pressure is definitely acting. The SI unit of stress BIBW2992 is the pascal Pa or N/m2. 1 Pa = 1 pN/μm2. Pressure exerted perpendicular to the surface of a material results in compressional or extension stress and pressure exerted parallel to the surface results in shear stress. Pressure. The magnitude of the pulling force. Pushes in the contrary path generate compression. For instance activation of myosin within a sarcomere generates stress at cell-cell or cell-tendon junctions. The potent force of gravity generates compression on cartilage and joints. Tension is normally.