Background The ideal healing technique for periodontal tissue problems would involve
May 21, 2019
Background The ideal healing technique for periodontal tissue problems would involve the functional regeneration of the alveolar bone, cementum, and periodontal ligament, with new periodontal attachment formation. animal experimental surgery conformed to the for of em Laboratory Animals /em . Postoperatively, the animals used their molars to chew food and the experiments did not affect the animals health. Results Cell tradition and mineralization After 3 days, main gingival fibroblasts emerged from your gingival cells (Number 1A). After differential culturing and subculturing, gingival fibroblasts were spindle-shaped with plentiful, standard cytoplasm, with large, round or oval nuclei in their centers (Number 1BC1D) and were 100% positive for vimentin (Number 1E, 1F). Open in a separate window Number 1 Gingival fibroblasts were cultured and subcultured (inverted microscopy, 100) (A, B). Gingival fibroblasts were fusiform with round nuclei (scanning electron microscopy [SEM], 700, 1000) (C, D). Gingival Sav1 fibroblasts were positive for vimentin (Polymer, 200) (E, F). After mineralization induction, fibroblasts showed mineralized nodules (inverted microscopy, 400) (G, H). The mineralized nodules are demonstrated Pexidartinib in a red color (Alizarin reddish, 400) (I, J) and crimson color (sterling silver nitrate, 400) (K, L). After mineralization, the fibroblasts demonstrated mineralized nodules (Amount 1G, 1H), that have been positive for alizarin crimson staining and metallic nitrate staining (Number 1IC1L). Two biomaterials Bio-Gide collagen membrane showed a good porous network structure with coarse materials (Number 2A, 2B). SIS showed a good porous network structure with thin materials (Number 2C, 2D). Open in a separate window Number 2 Bio-Gide collagen membrane showed a network structure with coarse materials (scanning electron microscopy [SEM], 500, 1000) (A, B). SIS scaffold showed a network structure with thin materials (SEM, 500, 1000) (C, D). The tissue-engineered periodontal membrane showed strenuous fibroblasts on Bio-Gide collagen membrane (SEM, 1000, 1500) (E, F). The tissue-engineered mineralized membrane displayed fibroblasts adhered on small intestinal submucosa (SIS) scaffold (SEM, 1200, 1500) (G, H). After mineralization-induction, the tissue-engineered mineralized membrane showed mineralized nodules (SEM, 2500, 3500) (I, Pexidartinib J). Sandwich tissue-engineered complex Investigation of the tissue-engineered periodontal membrane showed that fibroblasts grew vigorously and adhered closely to both sides of the Bio-Gide collagen membrane (Number 2E, 2F). The structure of the tissue-engineered mineralized membrane showed that fibroblasts adhered and were stretched fully on one side of the SIS and proliferated vigorously (Number 2G, 2H). After induction of mineralization, mineralized nodules were observed (Number 2I, 2J). The structure of the sandwich tissue-engineered complex included a tissue-engineered periodontal membrane between 2 tissue-engineered mineralized membranes. This is demonstrated schematically in Numbers 3AC3D. Open in a separate window Number 3 Schematic diagrams of the sandwich tissue-engineered complex. The tissue-engineered periodontal membrane (A). The tissue-engineered mineralized membrane (B, C). The sandwich tissue-engineered complex (D). Restoration of periodontal problems Periodontal defects were created in the right maxillary, right mandibular, and the remaining mandibular periodontal Pexidartinib cells (Number 4A, 4B). The periodontal problems were repaired using the tissue-engineered periodontal membrane in the periodontal membrane group, and by the sandwich tissue-engineered complex in the sandwich group (Number 4C, 4D). At 10 days after operation, all 6 beagle dogs survived and retained good health; all wounds experienced healed well (Number 4E, 4F). Six dogs in the 2 2 organizations (3 in each group) were sacrificed after 10 days and 20 days after operation, respectively. Open in a separate window Number 4 Periodontal cells of Pexidartinib the 2nd, 3rd, and 4th premolars of.