There is good evidence for impairment of spermatogenesis and reductions in
April 26, 2017
There is good evidence for impairment of spermatogenesis and reductions in sperm counts and testosterone levels in chronic alcoholics. by 13%. Multidirectional changes of the activities of testicular dehydrogenases were detected. We thus obtained complex assessment of chronic alcoholism effects in male gonads affecting especially amino acid protein ATP and NADPH metabolism. Our results demonstrated profound changes in testes on the level of proteome and genome. We suggest that the revealed metabolic disorders can have negative implication on cellular regulation of spermatogenesis under long-term ethanol exposure. for 5 min rinsed with 70% ethanol and air-dried. Pellets were dissolved in TBE buffer (10 mM Tris-HCl and 1 mM EDTA pH 8) and then fractionated through 2% agarose gels (50-60 V; 3.5 h). After electrophoresis the gels were stained with ethidium bromide and visualized under a UV transilluminator (BIORAD USA). Analysis INO-1001 of electrophoresis data was carried out with Quantity One Software (USA). The expression (ortholog of human (J?ger mRNA and β-actin mRNA contents. The obtained data were calculated and expressed as the mean ± standard error of the mean (mean±S.E.M.). Data were compared using Student’s t-test. Differences were considered to be statistically significant at mRNA expression was INO-1001 indicated in testes of rats with chronic alcoholism (Figure 2). This parameter decreased 3.5 times as compared with control. Figure 2 CYP3A2 mRNA in rat testes:a – electrophoregram of CYP3A2 and reference-gene β-actin RT-PCR products (arrows indicate appropriate DNA fragments); b – average rate of CYP3A2 mRNA expression in rat testes. * mRNA expression and protein content elevation in alcohol-treated rat testes with simultaneous spermatogenesis violations (Shayakhmetova expression in rat testes. CYP3A2 is the rat ortholog of the human enzyme CYP3A4 (J?ger mRNA level. Ethanol has been reported to be either an inducer or an inhibitor of CYP3A expression. CYP3A exposure induced P450 3A in primary cultures of human and rat hepatocytes (Kostrubsky mRNA level and CYP3A activity in a dose-dependent manner (Feierman studies indicated a relationship between CYP3A and the duration of ethanol exposure. In rats fed ethanol with the Lieber-DeCarli diet for 7-14 days STAT6 both ERND catalytic activities and immunoreactive CYP3A were increased (Roberts expression is highly regulated by pregnane X receptor (PXR) a member of the nuclear receptor superfamily regulating gene transcription in a ligand-dependent manner (Kliewer was demonstrated to be expressed in rat testes (Kim mRNA in the testes by ethanol could indicate its ability to affect at the transcription level independently of PXR. Findings in rodent models have shown that INO-1001 di-2-ethylhexyl phthalate is able to induce in testes and liver resulting in INO-1001 intensification of testosterone metabolism (16alpha- and 6beta-hydroxylation increase) (Kim mRNA expression could at least partially mediate the ability of ethanol to disturb testosterone metabolism and act as an endocrine disruptor. Our results on cholesterol content changes are in good accordance with other authors’ data demonstrating that chronic ethanol exposure causes significant increase in levels of testicular cholesterol free fatty acid phospholipids and triglycerides (Radhakrishnakartha mRNA expression and DNA fragmentation processes as well as changes in cholesterol and protein thiol group contents allowed us to obtain complex estimation of this pathologic influence in male gonads especially on the metabolism of amino acids proteins ATP and NADPH. Our results demonstrated profound INO-1001 changes in testes on the level of proteome and genome. We suggest that the revealed testicular metabolic disorders could have negative implications on cellular regulation of spermatogenesis under long-term ethanol exposure. Competing interests The authors declare that they have no competing interests. REFERENCES Adams ML Little PJ Bell B Cicero TJ. Alcohol affects rat testicular interstitial fluid volume and testicular secretion of testosterone and beta-endorphin. J Pharmacol Exp Ther. 1991;258:1008-1014. [PubMed]Aitken RJ Baker MA. Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development. Int J De Biol. 2013;57:265-272. [PubMed]Albano E. Alcohol oxidative stress and free radical damage..